Biology

• 1. Genomic Features
• 2. Subsystems of Cells
  • 2.1. Support and Movement System
  • 2.2. Energy Metabolism System
  • 2.3. mass metabolism system
  • 2.4. signalling system
  • 2.5. DNA operation system
• 3. RNA Sequencing
  • 3.1. Bulk RNA Sequencing
    • 3.1.1. RNA Isolation
    • 3.1.2. Library Preparation
    • 3.1.3. Sequencing
    • 3.1.4. Quality Control of Data
    • 3.1.5. Reads Mapping and Counting
    • 3.1.6. expressing matrix and its normalization
    • 3.1.7. Comparison between Samples
    • 3.1.8. de nove assembly for RNA
    • 3.1.9. alternative splicing
    • 3.1.10. new gene discovery
  • 3.2. Single Cell RNA Sequencing
    • 3.2.1. Single cell isolation
    • 3.2.2. Library Making
    • 3.2.3. Sequencing
    • 3.2.4. Data analysis
  • 3.3. Spatial RNA Sequencing
  • 3.4. Sequencing machines
    • 3.4.1. Illumina
  • 3.5. Library preparation kits
    • 3.5.1. Illumina
  • 3.6. Sequencing kits
    • 3.6.1. Illumina
  • 3.7. Reference
• 4. Software in Bioinformatics
  • 4.1. Read Quality of Sequencing
• 5. Experiment Language
• 6. Sex determination in Zebrafish
  • 6.1. Primary Sex Determination and Secondary Sex Determination
  • 6.2. Sex Determination Mechanism in Vertebrates
    • 6.2.1. Sex Determination in Mammals
    • 6.2.2. Sex Determination of Birds
    • 6.2.3. Sex Determination in Reptiles
    • 6.2.4. Sex Determination in Amphibians
    • 6.2.5. Sex Determination in Fish
  • 6.3. Sex Determination of Zebrafish
    • 6.3.1. Introduction
    • 6.3.2. Facts
  • 6.4. References
• 7. Reagents and Protocols
  • 7.1. Reagents
    • 7.1.1. Seawater
    • 7.1.2. EM3 reagent
  • 7.2. Protocols
    • 7.2.1. Nucleic Acids Extraction
    • 7.2.2. PCR Amplification